In Vitro Biological Activities and Relative Chemicals in Different Extracts of Licorice and Fermenting Licorice

Abstract

Purpose: This paper aims to qualitatively and quantitatively analyze the differences between the main chemicals in different licorice and fermented licorice (FL) extracts and their <i>in vitro</i> skin care activities and active ingredients. To evaluate the effect of fermentation on the efficacy of licorice.Methods: The DPPH free radical scavenging activity, mushroom tyrosinase activity, tyrosinase activity of B16F10 cells, and melanin synthesis inhibition activity of different extracts treated by fermentation and different extracts without fermentation treatment were evaluated. The effects of ethyl acetate components (EaF) including glycyrrhizin, isoglycyrrhizin, liquiritoside, isoliquiritigenin and glycyrrhizin ketone A on melanin synthesis were tested in vitro. Further assessment of FL at stromal metalloproteinase-1 (MMP-1) levels in human dermal fibroblasts (HDF).Results: Glycyrrhizin, isoglycyrrhizin, liquiritoside, isoliquiritigenin were improved in FL, and the antioxidant and MMP-1 inhibitory activities of FL controls were significantly increased.Conclusion: After fermentation, the anti-aging effect of licorice was greatly improved, indicating that the fermentation process had a good effect on improving the efficacy of licorice.