Abstract
.Abstract: In order to investigate the potential of Nigella sativa (N. sativa) oil as an active ingredient for whitening cosmetics, we prepared N. sativa oil. We measured its inhibitory effects on mushroom tyrosinase activity, cellular ty-rosinase activity, and melanin synthesis inhibitory activity in B16 melanoma cells. N. sativa oil and its components showed inhibitory activity against mushroom tyrosinase and mela nin synthesis. In a melanin synthesis inhibition assay using mouse B16-F10 melanoma cell, it reduced melanin productio n up to 86 % at a concentration of 10 mg/mL without cytotoxicity. In the study on the melanogenic protein e xpressions by using RT-PCR and Western blot, N. sativa oil and its components inhibited expression of tyrosinase prot ein, which is a well-known key protein on melano-genesis, and tyrosinase expression was gradually decreased in a dose-dependent manner. Therefore, this result sug-gests that N. sativa oil could be used as an active ingredient for whitening cosmeti cs.Keywords: skin-whitening, Nigella sativa, thymoquinone, thymohydroquinone , B16 melanoma
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