Abstract
BackgroundIn the present study, previously isolated, safe, and avirulent enterococci strains were exploited for their metabolic profile (Bhagwat et al., Asian J Pharm Clin Res 12: 2019). ResultsThirteen enterococci strains of human origin produced important enzymes like amylase (0.5–0.7 mg ml−1), protease (192–264 mg ml−1), lipase (8–10 mg ml−1), bile salt hydrolase, conjugated linoleic acid (CLA), and lactic acid (highest 12 mg ml−1), thus implicating potential attributes of starter cultures in food and dairy industry. Biogenic amines like arginine and tryptamine were produced after 4 days above 25 °C. Castor oil (highest yield 60 μg ml−1) and sunflower oil (highest yield 48 μg ml−1) both proved to be excellent sources of CLA production. Reduction assays using FRAP, ABTS (above 83%), and DPPH (30–50%) revealed excellent radical scavenging properties of cell-free supernatants of Enterococcus strains. ConclusionThe results implicate the future potential of application enterococci for therapeutic purpose as well as the food industry.
Highlights
In the present study, previously isolated, safe, and avirulent enterococci strains were exploited for their metabolic profile (Bhagwat et al, Asian J Pharm Clin Res 12: 2019)
Qualitative detection of enzymes All the enterococcal strains produced a zone of precipitation after adding iodine
Production of biogenic amines The strains were screened for the production of biogenic amines
Summary
Previously isolated, safe, and avirulent enterococci strains were exploited for their metabolic profile (Bhagwat et al, Asian J Pharm Clin Res 12: 2019). Results: Thirteen enterococci strains of human origin produced important enzymes like amylase (0.5–0.7 mg ml−1), protease (192–264 mg ml−1), lipase (8–10 mg ml−1), bile salt hydrolase, conjugated linoleic acid (CLA), and lactic acid (highest 12 mg ml−1), implicating potential attributes of starter cultures in food and dairy industry. The potential of the probiotic organism depends on the beneficial secondary metabolites produced by them. They are known to produce various enzymes like β-galactosidase, proteases, lipases, and bacteriocins that possess antimicrobial activity. Proteases and lipases help in the formation of biofilms, in turn, improving the process of pathogen exclusion. These enzymes help in reducing hypertension [2, 3]. The screening of probiotics considers metabolite production in their selection criteria [4, 5]
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