Abstract
Natural medicinal products possess diverse chemical structures and have been an essential source for drug discovery. Therefore, in this study, α-mangostin (AM) is a plant-derived compound was investigated for the apoptotic effect on human cervical cancer cells (HeLa). The cytotoxic effects of AM on the viability of HeLa and human normal ovarian cell line (SV40) were evaluated by using MTT assay. Results showed that AM inhibited HeLa cells viability at concentration- and time-dependent manner with IC50 value of 24.53 ± 1.48 µM at 24 h. The apoptogenic effects of AM on HeLa were assessed using fluorescence microscopy analysis. The effect of AM on cell proliferation was also studied through clonogenic assay. ROS production evaluation, flow cytometry (cell cycle) analysis, caspases 3/7, 8, and 9 assessment and multiple cytotoxicity assays were conducted to determine the mechanism of cell apoptosis. This was associated with G2/M phase cell cycle arrest and elevation in ROS production. AM induced mitochondrial apoptosis which was confirmed based on the significant increase in the levels of caspases 3/7 and 9 in a dose-dependent manner. Furthermore, the MMP disruption and increased cell permeability, concurrent with cytochrome c release from the mitochondria to the cytosol provided evidence that AM can induce apoptosis via mitochondrial-dependent pathway. AM exerted a remarkable antitumor effect and induced characteristic apoptogenic morphological changes on HeLa cells, which indicates the occurrence of cell death. This study reveals that AM could be a potential antitumor compound on cervical cancer in vitro and can be considered for further cervical cancer preclinical and in vivo testing.
Highlights
IntroductionHow to cite this article El habbash et al (2017), In vitro assessment of anti-proliferative effect induced by α-mangostin from Cratoxylum arborescens on human cervical cancer cells (HeLa) cells
Various tropical plants exhibit interesting biological activities for therapeutic applications; several new biologically active compounds may exert a synergetic anticarcinogenic effectHow to cite this article El habbash et al (2017), In vitro assessment of anti-proliferative effect induced by α-mangostin from Cratoxylum arborescens on human cervical cancer cells (HeLa) cells
The MTT assay results indicated that AM treatment markedly decreased the cell viability of the treated HeLa and Ca Ski cells compared with that of cells unexposed to AM
Summary
How to cite this article El habbash et al (2017), In vitro assessment of anti-proliferative effect induced by α-mangostin from Cratoxylum arborescens on HeLa cells. The main phytochemical compounds found in C. arborescens are xanthones, which exhibit various significant pharmacological properties (Sidahmed et al, 2013). AM acts as an antiparasitic, antidiabetic (Ibrahim et al, 2014b), anti-inflammatory (Chairungsrilerd et al, 1996), antioxidant (Márquez-Valadez et al, 2009), anti-tumor (Chitchumroonchokchai et al, 2013), antibacterial (Negi, Jayaprakasha & Jena, 2008; Sakagami et al, 2005), antifungal (Pedraza-Chaverri et al, 2008), cardio protective (Devi Sampath & Vijayaraghavan, 2007), anti-ulcer (Sidahmed et al, 2013) and can act as well as an anti-obesity agent (Ibrahim et al, 2015). Significant cytotoxic effect of AM has not been observed in cervical cancer cells; this study investigated the antitumor effect of this compound on cervical cancer cell line HeLa
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