Abstract
BackgroundVitrification is the main technique in the assisted reproductive technique (ART) labs. Sperm vitrification exposes sperm to damage. The aim of the present study was to evaluate the in vitro effect of Ceratonia siliqua (C. siliqua) application on sperm parameters and chromatin quality in normozoospermic aged men. Semen samples (n = 40) were collected from normozoospermic men over 45 years old. Each specimen was divided into four aliquots to form the subsequent groups: fresh (group I), vitrification without treatment (group II), vitrification with the medium supplemented by 20 μg/ml C. siliqua (group III), and vitrification with the thawing medium supplemented by 20 μg/ml C. siliqua (group IV). Sperm progressive motility, normal morphology and viability were assessed. Also, sperm chromatin quality was evaluated by aniline blue (AB), toluidine blue (TB), and sperm chromatin dispersion (SCD) staining.ResultsVitrification caused a significant decrease in sperm progressive motility, normal morphology and viability as well as chromatin quality compared to fresh samples (p < 0.05). Supplementation of vitrification/thawing medium with C. siliqua significantly improved sperm progressive motility, normal morphology, viability, and chromatin quality compared to vitrification without any supplementation (p < 0.05).ConclusionsThe study showed that C. siliqua can improve the detrimental effect of vitrification on sperm parameters and chromatin quality of normozoospermic aged men.
Highlights
Vitrification is the main technique in the assisted reproductive technique (ART) labs
Study design This study was approved by the Ethical Committee of Kermanshah University of Medical Sciences (IR.KUMS.REC.1397.259), and the written informed consent was obtained from every patient participating in the research
The percentage of the sperm progressive motility, normal morphology, and viability significantly decreased in all vitrified groups compared to fresh groups (p < 0.05)
Summary
Vitrification is the main technique in the assisted reproductive technique (ART) labs. The aim of the present study was to evaluate the in vitro effect of Ceratonia siliqua (C. siliqua) application on sperm parameters and chromatin quality in normozoospermic aged men. Male infertility is approximately estimated to be the cause of 50% of all infertility cases [1]. It is a concern since advanced paternal age may be related to increased abortion rates and different side effects on the newborn. Increased age might be related to reduced sperm quality [2]. Human sperm cryopreservation has been industrialized, turning to the main issue in assisted reproductive It was recently reported that the average paternal age is increasing, and multiple cultural and socioeconomic factors affect fatherhood delay [3].
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