In Vitro Antiviral Activities of Salinomycin on Porcine Epidemic Diarrhea Virus.

Chen Yuan,Penghao Zhang,Xintong Huang,Yichao Ma,Ruiyu Zhai,Anyuan Xu,Qian Yang

doi:10.3390/v13040580

Chen Yuan, Penghao Zhang + Show 5 more

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https://doi.org/10.3390/v13040580

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Journal: Viruses	Publication Date: Mar 30, 2021
Citations: 9	License type: CC BY 4.0

Affiliation: Nanjing Agricultural University

Abstract

Porcine epidemic diarrhea virus (PEDV), an enteropathogenic coronavirus, has catastrophic impacts on the global pig industry. Owing to the lack of effective vaccines and specific therapeutic options for PEDV, it is pertinent to develop new and available antivirals. This study identified, for the first time, a salinomycin that actively inhibited PEDV replication in Vero cells in a dose-dependent manner. Furthermore, salinomycin significantly inhibited PEDV infection by suppressing the entry and post-entry of PEDV in Vero cells. It did not directly interact with or inactivate PEDV particles, but it significantly ameliorated the activation of Erk1/2, JNK and p38MAPK signaling pathways that are associated with PEDV infection. This implied that salinomycin inhibits PEDV replication by altering MAPK pathway activation. Notably, the PEDV induced increase in reactive oxidative species (ROS) was not decreased, indicating that salinomycin suppresses PEDV replication through a pathway that is an independent pathway of viral-induced ROS. Therefore, salinomycin is a potential drug that can be used for treating PEDV infection.

Highlights

MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Citation: Yuan, C.; Huang, X.; Zhai, Abstract: Porcine epidemic diarrhea virus (PEDV), an enteropathogenic coronavirus, has catastrophic impacts on the global pig industry
Porcine epidemic diarrhea virus (PEDV) is a porcine enteropathogenic coronavirus whose infections are associated with high morbidity in swine of all ages, as well as a high mortality rate in suckling piglets, thereby negatively impacting the global pig industry [1,2,3]
Piglets infected with PEDV are characterized by vomiting, watery diarrhea and dehydration [4,5]

Summary

Introduction

MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Citation: Yuan, C.; Huang, X.; Zhai, Abstract: Porcine epidemic diarrhea virus (PEDV), an enteropathogenic coronavirus, has catastrophic impacts on the global pig industry. Salinomycin significantly inhibited PEDV infection by suppressing the entry and post-entry of PEDV in Vero cells. It did not directly interact with or inactivate PEDV particles, but it significantly ameliorated the activation of Erk1/2, JNK and p38MAPK signaling pathways that are associated with PEDV infection. Porcine epidemic diarrhea virus (PEDV) is a porcine enteropathogenic coronavirus whose infections are associated with high morbidity in swine of all ages, as well as a high mortality rate (up to 100%) in suckling piglets, thereby negatively impacting the global pig industry [1,2,3]. Antiviral drug therapy has been considered to be a novel and effective way for treating the infections associated with PEDV. The antiviral effects of salinomycin on PEDV infections have not been established

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