Abstract
Porcine epidemic diarrhea virus replication in duck intestinal cell line.
Highlights
We examined Porcine epidemic diarrhea virus (PEDV) replication in a newly established immortalized duck intestinal epithelial cell (MK-DIEC) line, which was generated from the intestinal tissues of a 19- day-old white Pekin duck embryo
We found that nearly 100% of the cells expressed aminopeptidase N (APN) on their surface
A) Twenty-four hours after infection, PEDV nucleoprotein in infected cells was detected by immunofluorescence assay using fluorescein isothiocyanate– labeled nucleoprotein-specific monoclonal antibody
Summary
We examined PEDV replication in a newly established immortalized duck intestinal epithelial cell (MK-DIEC) line, which was generated from the intestinal tissues of a 19- day-old white Pekin duck embryo. We used a rabbit polyclonal anti-human APN antibody (Abcam, Cambridge, MA, USA) in an indirect immunofluorescence assay (IFA) to examine whether MK-DIECs express APN. We found that nearly 100% of the cells expressed APN on their surface
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