In-vitro Antioxidant and Cytotoxic properties of Strobilanthes kunthianus

Abstract

Background: Strobilanthes kunthianus Nees T Anders (S. kunthianus, Neela kurinji) is a shrub in the grasslands of Western Ghats in India. The Nilgiris, which literally means the blue mountains got its name from the purplish blue flowers of Neelakurinji that blossoms gregariously once in twelve years. It is well known for its biological properties. However, so far there is no detailed antioxidant and cytotoxic studies has been carried out. Objective: The present study aimed to evaluate in-vitro antioxidant and cytotoxic activities of various parts of S. kunthianus extracts. Methods: Fourteen extracts were prepared in different parts of S. kunthianus including stem, leaves, flowers and roots. All the extracts were examined for in-vitro antioxidant and cytotoxic activities using standard methods. The total phenol content was estimated by Folin Ciocalteau method. Results: The crude methanol flower extract was found to contain very high total phenol content among all the extracts. Among the tested extracts, the ethyl acetate extracts of root and stem showed potent in-vitro antioxidant activity, when compared to ascorbic acid in ABTS method. These extracts showed better activity than other successive extracts of root and stem. In case of methanol extracts, the crude extracts showed potent antioxidant activity in ABTS, H2O2, and total antioxidant capacity methods. The crude methanol flower extract was found to be the most potent among the methanol extracts. Many natural products have served as anticancer agents in the treatment and also as lead compounds for further research. All the extracts were tested for their cytotoxic activity against Hep-2 and HeLa cell lines. But all the extracts showed weak activity in the tested cell lines. Conclusion: S. kunthianus merits further investigation in animal models to confirm its antioxidant and anticancer properties and to isolate the phytoconstituents that are responsible for their biological activities.