IN VITRO ANTIOXIDANT ACTIVITY, ANTIMICROBIAL AND PRELIMINARY CYTOTOXIC ACTIVITY OF CYNOMETRA RAMIFLORA- A MANGROVE PLANT

Sadia Afrin,Md Morsaline Billah,Farah Sabrin,Raihana Pervin,Md Hossain Sohrab,Md Emdadul Islam,Kazi Didarul Islam,Satyajit Roy Rony

doi:10.15414/jmbfs.2016.6.2.844-850

Abstract

A mangrove medicinal plant Cynometra ramiflora (Family: Leguminosae) was selected to investigate the bioactivities namely antioxidant, antimicrobial and preliminary cytotoxic activity using methanol and chloroform extracts of the leaves and stems, respectively. In 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, 50% inhibitory concentration (IC50) of the methanolic stem extract was found to be 31.62 µg.mL-1. Reducing power of the same extract demonstrated consistent increase in a concentration-dependent manner and was comparable with quercetin while ferric reducing antioxidant power (FRAP) assay revealed potential total antioxidant capacity (84.0 mM Fe (II)/g of extract). In addition, the presence of total phenolics (96.2 mg GAE/g of extract), total flavonoids (166.4 mg QE/g of extract) and tannins content (80.4 mg GAE/g of extract) were determined in the methanolic stem extract. The chloroformic stem extract exhibited moderate antimicrobial activity against a number of bacterial strains while the MIC values of extracts were in the range from 62.5 to 500µg.mL-1. The methanolic stem and leaf extracts demonstrated strong lethality in preliminary cytotoxicity assay using brine shrimp nauplii where the 50% lethal concentration (LC50) values were 1.596 and 4.613 µg.mL-1 respectively. It can be therefore concluded that the methanolic extracts of C. ramiflora possess potential antioxidant, antimicrobial and strong preliminary cytotoxic activity and could be further exploited for prospective scientific exploration towards bioactive principles.

Highlights

Medicinal plants are the imperative sources of indigenous medical systems (Ahmed et al, 2009) and they serve the primary health care needs of more than 80% of the people in the world (Hassan et al, 2009)
Grown C. ramiflora was collected from Dhangmaree, Chadpai Range of the Sundarbans East Division situated at Bagerhat district, Bangladesh on 16th December, 2011 and collected plant samples were sent to Bangladesh National Herbarium, Dhaka, Bangladesh for taxonomical identification
A number of methods are available for the determination of free radical scavenging activity but the DPPH assay employing the stable 2,2-diphenyl-1picryl-hydrazyl radical (DPPH) has received the maximum attention owing to its ease of use and its convenience (Concepcion et al, 1998)

Summary

Introduction

Medicinal plants are the imperative sources of indigenous medical systems (Ahmed et al, 2009) and they serve the primary health care needs of more than 80% of the people in the world (Hassan et al, 2009). The global efforts remain still invigorated by the numerous attempts to analyze bioactivity and search for bioactive compounds from plant sources. In this context, mangrove ecosystem offers a large number of relatively untapped species in an exclusive climatic condition with unique metabolic profile. Different extracts of C. ramiflora have been reported to have antioxidant (Bunyapraphatsara et al, 2003; Muhtadi et al, 2014), antihyperglycemic (Tiwari et al, 2008), cytotoxic (Uddin et al, 2011; Muhtadi et al, 2014), antibacterial and antinociceptive (Siraj et al, 2013) activity. We report the antioxidant, antimicrobial and preliminary cytotoxic activity of methanolic and choloroformic leaf and stem extract of C. ramiflora

Methods

Results

Conclusion