Abstract
The present study was aimed to investigate the phytochemical, antioxidant, antimicrobial activity of in vivo (root, stem and leaf), and in vitro derived leaf callus of Decalepis arayalpathra. The explants were cultured on MS medium supplemented with different concentration and combination of auxins and cytokinins for callus induction. The total phenolic, flavonoid and tannin contents in different solvent extracts were analysed using standard methods. Antibacterial and antifungal activity of different solvent extracts was carried out using the agar well diffusion method and food poison technique. Maximum callus induction was noticed on MS media supplemented with 2, 4-D (9.05µM/L) and BAP (4.44 µM/L) + NAA (2.69 µM/L) respectively. Total phenolic and flavonoid contents were higher in methanolic extracts of root and stem compared to other extracts. Antimicrobial activities was significantly higher in methanolic extract of root, leaf, stem and callus followed by aqueous and chloroform extract. The maximum antibacterial activity was observed in Klebsiella pneumoniae, Staphylococcus aureus and Escherichia coli in methanolic extracts of stem, leaf and callus. Our results demonstrated the phytochemical and antimicrobial activities of D. arayalpathra and further studies have to be carried out to identify the novel compounds.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
