In Vitro Antioxidant Activity and GC-MS Analysis of Sesbania grandiflora (L.) Pres. Leaves

Abstract

Sesbania grandiflora (L.) Pres. is a member of the Fabaceae family. In the DPPH antioxidant assay ethanol excerpts of August plant leaves scavenge more free radicals at volumes of 5 to 20 µl i.e., 16.33%, 19.88%, 23.07% & 27.59% as compared to other two solvents such as acetone and water. In the CUPRAC antioxidant assay ethanol excerpts also scavenge more free radicals at concentrations of 2, 3 4 and 5 µg/1000µl i.e., 5.15%, 8.68%, 13.82% & 17.17 as compare to the other two solvents such as acetone and water. In GC-MS analysis 42 compounds are pointed out by chromatogram image. Out of 42 compounds 15 compound are antioxidant in nature such as 1-Tetradecene, 1-HEXADECENE, 1-OCTADECENE, 1-Nonadecene, Phytol, DOCOSANE, 1-HYDROXY-2,2,6,6-TETRAMETHYL-3-(1-PIPERIDINY, Pentatriacontane, 1-Heptacosanol, Stigmasterol, 9,19-Cyclolanostan-3-ol, 24-methylene-, (3.beta.)-, Neophytadiene, n-Hexadecanoic acid, .alpha.-Tocospiro B & Squalene which justify these plant leaves are antioxidant in nature. 8 antimicrobial compounds; 1-Tetradecene, 1-HEXADECENE, 8-Pentadecanone, 1-Nonadecene, DOCOSANE, Squalene, n-Hexadecanoic acid & Heneicosane, 5 anti-inflammatory compound; Neophytadiene, n-Hexadecanoic acid, Phytol, .alpha.-Tocospiro B & Stigmasterol, 4 anticancer compound; 1-OCTADECENE, Vitamin E, .gamma.-Sitosterol & Squalene, 1 anti-neuropathic cholesterolytic compound: MOME INOSITOL, 2 antitumor compound; .beta.-Amyrin & .alpha.-Amyrin and 4 antidiabetic compound; .gamma.-Sitosterol, Vitamin E , .alpha.-Tocospiro B & 9,19-Cyclolanostan-3-ol, 24-methylene-, (3.beta.)- are also identified which shows these plant is immense potential to make an individual place on medical science for production of plant based medicine. The main manifesto of this investigation is to find out Phyto-therapeutic antioxidant which has extreme benefits and limited or no side effects.