Abstract

Objective: The objective of this study was to carry out the antioxidant potential of Elaeagnus conferta (EC) Roxb. leaves that were worked on various assay 2,2-diphenyl-1-picrylhydrazyl hydrate (DPPH) and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation decolorization, total reducing power was studied by ferric reducing antioxidant power (FRAP) and total radical trapping antioxidant potential (TRAP) method. The spectroscopic technique Fourier transform infrared (FTIR) spectrophotometer was performed to detect the characteristic peak values and their functional groups of EC Roxb. 
 Methods: Ethanol solvent is used ABTS, DPPH, FRAP, TRAP, and FTIR analysis.
 Results: ABTS assay, IC50 value was found to be 60 μg/ml compared with standard ascorbic acid 64 μg/ml. DPPH IC50 value of EC Roxb. compared with standard ascorbic acid was found to be 0.43 μg/ml and 0.62 μg/ml. FRAP IC50 value along with standard ascorbic acid was found to be 0.582 μg/ml and 0.596 μg/ml. The IC50 value of TRAP along with standard ascorbic acid was found to be 0.39 μg/ml and 0.62 μg/ml. In FTIR spectroscopic study, it revealed different characteristic peak values with various functional compounds such as phenols, alcohols, amide, alkanes, carboxylic acids, alkenes, primary amines, aromatics, ester, alkyl halides, and aliphatic amines compounds.
 Conclusion: EC Roxb. leaves have a potent rich source of antioxidant. In FTIR analysis, it is confirmed with the presence of phenolic compounds that can be emphasized for efficacy in pharmaceutics.

Highlights

  • Over 175,000 cancers are expected each year globally

  • In vitro free radical scavenging activity of Elaeagnus conferta (EC) Roxb. leaf extract 2,2’-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay EC Roxb. leaves of ethanol extract were using ABTS+ solution, it was followed by reference of Re et al [10]

  • EC Roxb. leaves of ethanol extract on ABTS+ are shown in Fig. 1, ascorbic acid used as a reference

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Summary

Introduction

Over 175,000 cancers are expected each year globally. Trends in cancer survival differ depending on the regions. Is a vast majority of the species that are abundantly found in subtropical regions of Asia It nearly consists of about 50–70 species that are found in the family of Elaeagnaceae. To identify the chemical structure for the phytoconstituents, Fourier transform infrared (FTIR) analysis is used to get an idea on various functional groups, the chemical bonds in a molecule can be determined, that is, responsible for their medicinal purposes [9]. This subject has focused on free radical scavenging assay and analysis of FTIR of the plant EC Roxb. This subject has focused on free radical scavenging assay and analysis of FTIR of the plant EC Roxb. leaves to identify those constituents responsible for the beneficial effects

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