In vitro antimicrobial study of Aspergillus flavus mycelial extract against different bacterial and fungal pathogenic strains

Abstract

The aim of our research was to determine the antimicrobial activity of Aspergillus Flavus against some selected bacterial and fungal pathogen. The research work was done in the laboratory of Agricultural Chemistry, Department of Agricultural Chemistry, Agriculture University Peshawar, Khyber Pakhtunkhwa, Pakistan during the month of May 2013. The agar well diffusion method was used. The Acetonitrile and n-hexane extracts were used. The result of both the extracts of Aspergillus flavus was found to be effective against all the tested Bacterial i.e. P. aeruginosa, E. coli, S. aureus, S. aureus (Methicillin resistant), S. aureus (Vancomycin resistant), and fungal pathogen A. niger, A. oryzae, C. albican, P. distalatum, F. oxysporum. The result in Acetonitrile solvent was quite good from the result in n-hexane fraction, which confirmed that the secondary metabolites are more soluble in acetonitrile. Minimum inhibitory concentration (MIC), of the extracts against these bacterial and fungal strains were in the range of 0.20 mg/ml. Different phytochemical analysis result indicate the presence of secondary metabolites like mycotoxin, Aflatoxin, Kojic acids which may be responsible for antimicrobial properties. From our result is the concluded that extract of Aspergillus flavus have potential against fungal and bacterial strains.