IN VITRO ANTIMICROBIAL ACTIVITY OF COCONUT SHELL LIQUID SMOKE AGAINST Candida albicans AND Lactobacillus acidophilus ATTRIBUTING TO ENDODONTIC TREATMENT FAILURE

Abstract

L. acidophilus bacteria and C. albicans fungi are the most common microorganisms isolated from the oral cavity of patients suffering from post-treatment of root canal endodontic infections. Chlorophenol Camphor menthol (ChM) is one of the antimicrobials used for root canal sterilizer but it still has many flaws. Coconut shell liquid smoke (CS-LS) is a natural product that has an antimicrobial activity and non-toxic chemical compounds for humans. This study aims at determining the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of L. acidophilus bacteria and C. Albicans fungi and comparing the antimicrobial activity between CS-LC with commercial root canal sterilization. The MIC and MBC of CS-LS were measured using 10 different concentrations ranging from 10-100%. MIC was determined by the broth dilution method. All concentration was cultured on each agar media to determine its MBC. The data were analyzed using the comparative test of Kruskall-Wallis with post-hoc Mann-Whitney. MIC and MBC of C. albicans were 10% of CSLS. Meanwhile, L. acidophilus has the MIC with a 50% concentration of CSLS and MBC with a 60% concentration of CS-LS. No significant difference in CS-LS and ChKM was found. Thus, CS-LS has a potential for endodontic treatment failure. Further research should be conducted to know other potential uses of CS-LS in dentistry.