In Vitro anti-diabetic and antioxidant potential of endophytic fungi isolated from Bryophyllum pinnatum L.

Abstract

Diabetes is a chronic metabolic disease and one of the leading causes of death compared to all health issues. Diabetes drugs can be used to regulate the activity of metabolic enzymes including amylase in diabetic patients. Endophytes are a group of microorganisms that reside in the internal tissue of all higher plants without causing any of the symptoms they live in. Endophytic fungi isolated from plants can serve as a reservoir of secondary metabolites since they can prevent diabetes through alpha-amylase activity. Current research has isolated endophytic fungi from the leaves of Bryophyllum pinnatum and extracts of dichloromethane (DCM), ethyl acetate (EA), butanol (BuOH) and methanol (MeOH) were carried out to study antioxidant and antidiabetic activities. Twenty-two (BPL1-BPL22) endophytic fungi were isolated from the leaves and their phytochemical presence was examined. Based on the presence of phytoconstituents, 11 endophytic fungi were chosen to analyze their radical scavenging and antidiabetic activities. In a radical scavenging assay, the fungal crude extracts showed a dose-dependent activity. The fungal crude extracts were determined for their antidiabetic potential via alpha-amylase and glucose uptake by yeast cell studies. The maximum alpha inhibitory activity was shown by DCM, EA and MeOH extracts of BPL20 isolate of about 52.03±0.31, 52.72±0.81 and 57.67±0.66 respectively. The glucose uptake by yeast cells by fungal crude extracts was evaluated and MeOH extracts of BPL20 showed 54.27±0.19% of glucose uptake at 500 μg/ml concentration. The results revealed the hypoglycemic activity of fungal crude extracts.