In vitro anticancer evaluation of chitosan/biogenic silver nanoparticle conjugate on Si Ha and MDA MB cell lines

Abstract

Nanomedicine is an innovative approach to cancer therapy. The present study is in vitro anticancer evaluation of chitosan-stabilized biogenic silver nanoparticles (Ch/Bio-AgNPs) on Si Ha (human cervical carcinoma) and MDA MB (human adenocarcinoma) cells. IC50 values of Bio-AgNP and Ch/Bio-AgNP were studied by MTT assay, antiproliferative and cell apoptosis-inducing activities of the conjugate were examined by ethidium bromide/acridine orange double staining, RO analysis, DNA fragmentation, Caspase 7 and 9 assays and flow cytometry. Gene expression was studied by real-time PCR using the β-actin housekeeping gene. As a result, on MDA MB cells IC50 value for Bio-AgNP and Ch/Bio-AgNP was 4.346 ± 0.6381 µg/ml and 0.9851 ± 0.0065 µg/ml, respectively, and on Si Ha cells it was 24.35 ± 1.390 µg/ml and 2.86 ± 0.319 µg/ml, respectively. Ch/Bio-AgNPs was a potent inhibitor on MDA MB and Si Ha cell lines with characteristic apoptosis and up-regulation of p53 and p38 genes.