In Vitro Anticancer Activity of Canthium parviflorum Lam. Extracts Against Cancer Cell Lines

Abstract

In this study, the methanol extracts of Canthium parviflorum leaf, leaf-derived callus, and root extracts were evaluated for the anticancer activity against the Caco2 human epithelial colorectal adenocarcinoma cell lines and liver cancer (HepG2) cell lines were used to screen the in vitro anticancer screening with the C. parviflorum extracts. The concentration ranges from 5 to 100 μg of C. parviflorum crude extracts were screened against Caco2 and HepG2 cells. The antiproliferative activity of C. parviflorum was performed by 3-[4,5-Di methyl thiazol-2-yl]-2,5-diphenyltetrazolium Bromide (MTT) assay. The cytotoxicity was highly significant in the Caco2 cell lines using the methanolic leaf callus extracts; comparatively leaf callus extracts showed potent cytotoxicity than the leaf extracts of the C. parviflorum; finally the leaf, leaf callus extracts, and root extracts showed maximum cytotoxicity against Caco2 and HepG2 cancer cell lines.