In Vitro Antibacterial Activity of Some Natural and Trade Iraqi Honey against MRSA Staphylococcus Heamolyticus Isolated from Some Burned Patients in Misan City

Abstract

The study is aimed to evaluate the antibacterial activity of both natural Iraqi honeys (Sidr, Eucalyptus) and trade honey sample against MRSA Staphylococcus heamolyticus isolated from burned patients in alsader hospital-misan city then were analyzed between (October to December in 2015). The samples were taken in order to determine the bacterial profile and antibiotic susceptibility. The isolates of Staphylococcus heamolyticus were tested against 5 different antibiotics by a disk diffusion method 100% of the Staphylococcus heamolyticus isolates were resistant to the Optochin, Lincomycin, Ampicillin, Amphotercin, while the inhibitory effect of Amikacin antibiotic were (24mm) on MRSA Staphylococcus heamolyticus. Total antibacterial activity was evaluated by measuring the clear zone around the well. Honey samples were tested in different concentration (75%, 50 %, 25%, 10% and 100%). However, the results showed the absences of inhibitory zone to both natural and trade honeys type on MRSA Staphylococcus heamolyticus tested bacteria after dilution of(25% Sidr honey sample, 50% Eucalyptus honey sample, 50% Trade honey sample), while Sidr honey sample have more effective in 75%v/v concentration against MRSA Staphylococcus heamolyticus tested bacteria. The minimum inhibitory concentration (MIC) of the Sidr honey sample were also determined. Finally cytotoxicity evaluating toward human RBC, the results revealed the Iraqi honeys sample have not any cytotoxicity in all concentration. The good antimicrobial potency of sidr and Eucalyptus Iraqi honeys could potentially be used as therapeutic against MRSA Staphylococcus heamolyticus as an alternative to the costly antibiotics.