In Vitro Antiaging Analysis of Topical Pharmaceutical Preparation Containing Mixture of Strawberry Fruit, Pomelo Peel, and Langsat Fruit Extracts

Abstract

The previous research has discovered that strawberry fruit, pomelo peel and langsat fruit peel have antioxidant activity. This research aimed to put the extract combination into several formula of gel and cream. Extract will be formulated into two gels with Natrium Carboxyl Methyl Cellulose as gelling agent for Formula 1 and Synthalen for Formula 2. Whereas there are three cream formulations with Glyceril Mono Stearat as surfactant for Formula 3, Polawax for Formula 4, and Simulsol 165 for Formula 5. Natrosol HBr was added to Formula 3 as thickening agent and Keltrol CG-SFT added to Formula 5. After five formulas being made, their stability was tested using climatic chamber within 30 days. Physical stability parameters were spreadability, stickiness, pH, viscosity, separation degree, and syneresis test. The chemical properties evaluation of the formula was done by measuring radical 2,2-difenyl-1-pikrylhidrazil (DPPH) scavenging activity percentage, anti-collagenase activity, anti-tyrosinase activity, and anti-elastase activity. The results showed that all of formulas have good activity in scavenging DPPH radical, anti-elastase, anti-collagenase and anti-tyrosinase as well. In addition, all formulas have good stability. There is no change in appearance after three times freeze-thaw cycle. Therefore, it was concluded that all formula can be developed as topical preparation for antiaging.