In Vitro Anti-Biofilm Activity of Bacteriophage K (ATCC 19685-B1) and Daptomycin against Staphylococci.

Maria Plota,Iris Spiliopoulou,Sophia G Antimisiaris,Nikolaos Giormezis,Eleni Sazakli,Michalis Leotsinidis,Fevronia Kolonitsiou,Foteini Gkartziou

doi:10.3390/microorganisms9091853

Maria Plota, Iris Spiliopoulou + Show 6 more

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https://doi.org/10.3390/microorganisms9091853

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Abstract

The purpose of the present study was to investigate anti-staphylococcal activity of daptomycin and bacteriophage K, alone or in combination, against biofilm-producers and non-producers S. aureus and S. epidermidis strains, under biofilm forming and cells’ proliferation conditions. Daptomycin and bacteriophage K (ATCC 19685B1), in different concentrations, were tested against 10 Staphylococcus aureus and 10 S. epidermidis, characterized by phenotypes and genotypes. The quantitative microtiter plate (crystal violet, CV), methylthiazoltetrazolium (MTT), and growth curve (GC) assays were performed. No statistically significant difference was found between species, whereas daptomycin alone performed better using medium and high concentrations of the drug and bacteriophage K was more active against strains with higher susceptibility, by CV and MTT assays. Best results were achieved using both agents combined in high concentrations. Bacteriophage K was effective within 3.8 and 2.4 h, depending on the concentration used, by the GC assay. Combination of daptomycin with bacteriophage K was more effective against staphylococci, depending on the concentrations used and strains’ susceptibility. Further studies are needed to evaluate if this approach might be a choice for prevention or therapy of biofilm-associated infections.

Highlights

In recent years major advances have been made in the use of medical devices such as prosthetic joints, artificial implants, contact lenses, peritoneal dialysis, urinary and central venous catheters, endotracheal tubes, mechanical heart valves, and pacemakers
Biofilm formation is based on the attachment, proliferation and maturation of bacteria embedded in a matrix of extracellular polymeric substances produced, and a final step of cells’ detachment [2]
In the present study we have focused on the third intervention, testing the activity of daptomycin and bacteriophage K against S. aureus and S. epidermidis clinical strains

Summary

Introduction

In recent years major advances have been made in the use of medical devices such as prosthetic joints, artificial implants, contact lenses, peritoneal dialysis, urinary and central venous catheters, endotracheal tubes, mechanical heart valves, and pacemakers. Even though this modern medical technology plays an increasingly important role in the healthcare system, despite all precautionary strategies applied in hospitals to prevent infections, medical devices are vulnerable to contamination and infection [1].

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