Abstract
Background: Antimicrobial resistance is the main concern worldwide to combat infectious. Over the years studies on leaf extracts Tinospora cordifolia have demonstrated the potent role its antibacterial property. The current study is an attempt to test its antibacterial property against Escherichia coli cell division.
 Material and methods: Phytochemical screening assay of T. cordifolia leaf extract was done using standard procedure and the results showed the presence of alkaloid, carbohydrate, terpenoid, steroid, tannin, amino acid, flavonoid and glycoside components.
 Results: HPLC analysis revealed the presence of berberine in T. cordifolia leaf extract. Further E. coli cells were treated with berberine to study its efficacy in inhibiting cell division. Antibacterial assay was performed by using disc diffusion method.
 Conclusion: Among aqueous, methanolic, ethanolic, chloroform, hexane and acetone extract only methanolic extract showed zone of inhibition.
Highlights
Microorganisms, like any other life form, tend to evolve over time
Phytochemical screening assay of T. cordifolia leaf extract was done using standard procedure and the results showed the presence of alkaloid, carbohydrate, terpenoid, steroid, tannin, amino acid, flavonoid and glycoside components
HPLC analysis revealed the presence of berberine in T. cordifolia leaf extract
Summary
Microorganisms, like any other life form, tend to evolve over time. Survival is the key for existence and it must adapt to its surrounding. Natural compounds present in medicinal plants enhancethe immune status and maintain disease free state [2]. T. cordifolia is one of the most widely used herb in Ayurveda, one of the ancient systems of medicine practiced in India It belongs to Menispermaceae family and commonly called heart leaved moonseed plant in English, Guduchi in Sanskrit, Giloe in Hindi, Teppatige in Telugu [3]. Fruits aggregate of T. cordifolia belongs to different classes of alkaloids, steroids, glycosides, diterpenoid lactones, sesquiterpenoids, phenolics, aliphatic compound [4,5]. These compounds are present in all parts of T. cordifolia but its concentration is more in root, stem and leaves. The current study is an attempt to test its antibacterial property against Escherichia coli cell division
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