In vitro anthelmintic activity of the extract of coffee husk fermented with Pleurotus ostreatus for Ascaridia galli

Irma Badarina,Heru Dwi Putranto,Endang Sulistyowati

doi:10.20884/1.jap.2017.19.1.595

Abstract

The objective of this research was to evaluate the anthelmintic efficacy of the ethanolic and aqueous extract of coffee husk fermented by Pleurotus ostreatus against Ascaridia galli. In vitro study of anthelmintic activity was conducted by counting the number of paralyzed worm body of Ascaridia galli during 24 hours in petri dish containing different levels of ethanolic and aqueous extract of fermented coffee husk, namely 0%, 1%, 2%, 3% and 4% (w/v) and compared to piperazine 0,5% (w/v) and NaCl 0,9%. The powdered samples of fermented coffee husk were macerated in the 30% ethanol solution with the ratio samples to solution = 1:3. In aqueous extract, samples to solution ratio was 1 : 7. The extract of fermented coffee husk possess phytochemical compunds which responsible for anthelmintic activity. The anthelmintic activity of ethanolic extract was better than aqueous extract. Fermented coffee husk ethanolic extract exhibited mortality of A.galli at concentration 2% while showed highest mortality at concentration 4%. Fermented coffee husk ethanolic extract have the potential to aid in the control of gastro intestinal parasites in animal

Highlights

Gastro intestinal parasites (GIP) have direct effects on and remain one of the main problems to productivity of the livestock
The common control mode of GIP based on the use of anthelmintics is strongly questioned because of the increasing development of resistance and residual effect to animal product (Wolstenholme et al, 2004; Sissouma et al, 2011)
One of the mushrooms is Pleurotus ostreatus (P. ostreatus) containing a number of active compounds that help as health-enhancers, potential against diseases and as biological response modifiers (Wong et al, 2011; Patel et al, 2012)

Summary

Introduction

Gastro intestinal parasites (GIP) have direct effects on and remain one of the main problems to productivity of the livestock. The common control mode of GIP based on the use of anthelmintics is strongly questioned because of the increasing development of resistance and residual effect to animal product (Wolstenholme et al, 2004; Sissouma et al, 2011). One of the mushrooms is Pleurotus ostreatus (P. ostreatus) containing a number of active compounds that help as health-enhancers, potential against diseases and as biological response modifiers (Wong et al, 2011; Patel et al, 2012). Ganeshpurkar et al (2012) reported that there was anthelmintic potential of Pleurotus florida to Ascaridia galli. The content of bioactive compounds from mushroom may be responsible for anthelmintic action. P. ostreatus was the same genus with Pleurotus florida, so it is expected to have anthelmintic activity

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