In vitro anthelmintic activity of Euphorbia forskalii J. Gay aqueous extracts evaluation on different life stages of Haemonchus contortus.

Abstract

The use of medicinal plants in the control of gastrointestinal parasitosis is a promising solution for improving the productivity of sheep flocks. In order to evaluate the anthelmintic activity of Euphorbia forskallii, in vitro bioassays were performed on three life stages of Haemonchus contortus. Five aqueous extracts concentrations namely 10mg/mL; 5mg/mL; 2.5mg/mL; 1.25mg/mL and 0.62mg/mL were used for adult worm mortality tests. Egg hatch inhibition and L3 larval migration inhibition tests were studied at 5mg/mL; 2.5mg/mL; 1.25mg/mL; 0.62mg/mL and 0.31mg/mL. A negative control PBS and a positive control levamisole 2.5mg/mL were established for each test. A phytochemical screening was performed to determine the presence of some secondary metabolites. The results obtained showed the presence of total polyphenols, total flavonoids and condensed tannins within the aqueous extracts of E. forskalii. A high and significant (P<0.05) morality rate compared to the negative control with an LC50 of 2.30mg/mL was obtained. Inhibition of egg hatch and larval migration were high and significant (p<0.05) compared to the negative control. There was an IC50 of 1.03mg/mL and 0.92mg/mL respectively for inhibition of egg hatching and L3 larval migration. The present study revealed the in vitro anthelmintic activity of E. forskalii aqueous extracts and allows us to consider in perspective complementary studies to confirm this activity.