In vitro and Insilico cytotoxicity activity of Aconitum Heterophyllum Phyto niosomes and its ethyl acetate root extract

Abstract

Niosomes as a novel drug delivery system is a nonionic surfactant-based vesicle, have attracted much attention in pharmaceutical fields due to their excellent behavior in encapsulating both hydrophilic and hydrophobic agents. In the present study a comparative free radical scavenging and anti-proliferative potential of ethyl acetate root extract of Aconitum heterophyllum (EAEAH) and Aconitum heterophyllum roots loaded phyto-niosomes (nEAEAH) were investigated. Initially the EAEAH and nEAEAH were prepared and their antioxidant potential were studied in terms of DPPH, Super oxide, Hydroxyl radical scavenging by employing the In-vitro method. Assessment of anti-proliferative activity of these extracts was done using MCF-7 cancer cell lines (human breast cancer) by employing MTT assay and trypan blue exclusion assay. In-silico docking was performed using Auto Dock Vina software to identify the suitable antagonistic ligand which can inhibit the proteins BRCA1, BRCA2 and HER2 to exert the cytotoxic effect. In the present study a concentration dependent antioxidant, and cytotoxic activity was observed for both the extracts. IC50 values were calculated for MTT and trypan blue assay and the nEAEAH showed 41.71μg/ml, 49.36μg/ml respectively, whereas for EAEAH it was found to be 54.89 and 67.13μg/ml.