Abstract
Cichorium intybus commonly known as chicory is a member of the family Compositeae. It has been known for various pharmacological activities and anticancer effects against various cell lines including breast cancer MCF-7, prostate cancer LNCaP, amelanotic melanoma C32 and renal adenocarcinoma ACHN. In the present study, aerial parts of C. intybus were collected, identified, soaked in n-hexane non polar solvent, filtered and evaporated till the extract residue was isolated, dried and stored at 4°C and then checked for cytotoxic activities. Lymphoblastic leukemia cells (Jurkat cells) were used to evaluate the cytotoxic effects of n-hexane extract of C. intybus. Extracts were added to the cultured cells of selected cell line in various concentrations (10, 25, 50, 75 and 100 µg/ml) and incubated for 24 h. Trypan blue exclusion assay, MTS assay and FACS analysis were carried out to analyze cell viability, cell proliferation and apoptosis, respectively. The non polar n-hexane extract significantly reduced the number of viable cells and cell proliferation percentage but induced the apoptosis haphazardly. Results of this study demonstrate that n-hexane extract of C. intybus has potent anti-proliferative and cytotoxic activity against Jurkat cells, a human leukemia cell line. Key words: Cichorium intybus, Jurkat cells, lymphoblastic leukemia cells, MTS, trypan blue assay, apoptotic analysis.
Highlights
Cichorium intybus is a small herb (Keshri et al, 1998) known as chicory belonging to the familyCompositeae (Pushparaj et al, 2007) or Asteraceae (Shaikh et al, 2012)
The objective of the present study was to evaluate the cytotoxic effect of n-hexane extracts of whole plant of C
The plant was put in macerating flask with ample non polar solvent n-hexane for 5 days with occasional shaking within mixture
Summary
Cichorium intybus is a small herb (Keshri et al, 1998) known as chicory belonging to the familyCompositeae (Pushparaj et al, 2007) or Asteraceae (Shaikh et al, 2012). Cichorium intybus is a small herb (Keshri et al, 1998). Known as chicory belonging to the family. Compositeae (Pushparaj et al, 2007) or Asteraceae (Shaikh et al, 2012). It widely exists in Mediterranean region, Iran, Europe and Northern Asia (Amirghofran et al, 2000) and present in Punjab, New Frontier and Hyderabad (Keshri et al., 1998). C. intybus is one of the plants cited by Hadith (Wani et al, 2011) and traditionally used in many indications. Its roots and leaves are used as bitter tonic cholagogue, cardio protective, diuretic, laxative, antihypertensive, anti-rheumatic and anti-diabetic agent.
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