In Vitro and In Vivo Photoprotective Effects of (-)-Loliode Isolated from the Brown Seaweed, Sargassum horneri.

Lei Wang,Jiachao Xu,Xiaoting Fu,In-Kyu Yeo,Hyun-Soo Kim,K K Asanka Sanjeewa,Jun-Geon Je,Jae-Young Oh,You-Jin Jeon,Ginnae Ahn,Caoxing Huang,Xin Gao

doi:10.3390/molecules26226898

Abstract

Skin is the largest organ of humans. Overexposure to ultraviolet (UV) is the primary environmental factor that causes skin damage. The compound, (-)-loliode, isolated from the brown seaweed Sargassum horneri, showed strong antioxidant and anti-inflammatory activities in in vitro and in vivo models. To further explore the potential of (-)-loliode in cosmetics, in the present study, we investigated the photoprotective effect of (-)-loliode in vitro in skin cells and in vivo in zebrafish. The results indicated that (-)-loliode significantly reduced intracellular reactive oxygen species (ROS) level, improved cell viability, and suppressed apoptosis of UVB-irradiated human keratinocytes. In addition, (-)-loliode remarkably attenuated oxidative damage, improved collagen synthesis, and inhibited matrix metalloproteinases expression in UVB-irradiated human dermal fibroblasts. Furthermore, the in vivo test demonstrated that (-)-loliode effectively and dose-dependently suppressed UVB-induced zebrafish damage displayed in decreasing the levels of ROS, nitric oxide, lipid peroxidation, and cell death in UVB-irradiated zebrafish. These results indicate that (-)-loliode possesses strong photoprotective activities and suggest (-)-loliode may an ideal ingredient in the pharmaceutical and cosmeceutical industries.

Highlights

The results indicated that SSQC4 effectively reduced intracellular reactive oxygen species (ROS) lev- intracellular ROS
To further investigate the bioactivity of (-)-loliode and to explore its potential in the cosmeceutical industry, in the present study, we investigated the photoprotective effect of (-)-loliode in in vitro and in vivo models
These results indicated that (-)-loliode effectively protected HaCaT cells against oxidative damage induced by UVB irradiation

Summary

Introduction

Skin is the largest organ and the first defensive line of the natural defensive system of the human body. Ultraviolet (UV) is the primary environmental factor that causes skin damage [1]. UV can be classified into three subtypes, including. UVA (320–400 nm), UVB (280–320 nm), and UVC (100–280 nm). UVB is characterized as causing more damage to human skin than UVA and UVC. This is because of its ability to penetrate the layers of the stratum corundum and epidermis [2]. UVB irradiation could cause skin damage such as thickening of the epidermis, pigmentation disorders, loss of elasticity, erythema, deep wrinkles, and skin cancer.

Methods

Results

Conclusion