Abstract
Abstract Pomegranates is abundant in polyphenols and is well-known for its antioxidant activity. Punicalagin (PG) is a major polyphenolic compound in the pomegranate peel. In certain conditions, PG can be hydrolyzed to punicallin (PL) and ellagic acid (EA), and PL can be further hydrolyzed to EA. PG, PL, and EA all play important roles in the antioxidant activity of pomegranate peels. This study was conducted to compare the in vitro antioxidant activity and in vivo anti-oxidative stress effects of PG, PL, and EA. For the in vitro test, 2,2-diphenyl-1-picrylhydrazyl radical (DPPH·) and superoxide anion ( O 2 · ¯ ) scavenging capacities, ferric-reducing antioxidant power (FRAP), and lipid peroxidation (LPO) inhibition capacities of PG, PL, and EA were tested. For the in vivo test, oxidatively stressed mice, which were induced by oxidized fish oil, were administrated PG, PL or EA (10 mg kg−1 d −1) for 21 days. The results showed that the in vitro antioxidant activity trends were EA>PG>PL>Trolox in scavenging DPPH·, PG>PL>EA≈Trolox in scavenging O 2 · ¯ , EA>PG≈PL>Trolox in FRAP, and Trolox>PG>EA>PL in LPO inhibition. In the in vivo test, the EA treatment increased the average daily weight gain and total antioxidant capacity (T-AOC) in the plasma (P
Published Version
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