Abstract
BackgroundGlow discharge plasma (GDP) procedure is an effective method for grafting various proteins, including albumin, type I collagen, and fibronectin, onto a titanium surface. However, the behavior and impact of titanium (Ti) surface modification is yet to be unraveled.PurposeThe purpose of this study is to evaluate and analyze the biological properties of fibronectin-grafted Ti surfaces treated by GDP.Materials and MethodsGrade II Ti discs were initially cleaned and autoclaved to obtain original specimens. Subsequently, the specimens were GDP treated and grafted with fibronectin to form Ar-GDP (Argon GDP treatment only) and GDP-fib (fibronectin coating following GDP treatment) groups. Blood coagulation test and MG-63 cell culture were performed to evaluate the biological effects on the specimen.ResultsThere was no significant difference between Ar-GDP and GDP-fib groups in blood compatibility analysis. While in the MTT test, cellular proliferation was benefited from the presence of fibronectin coating. The numbers of cells on Ar-GDP and GDP-fib specimens were greater than those in the original specimens after 24 h of culturing.ConclusionsGDP treatment combined with fibronectin grafting favored MG-63 cell adhesion, migration, and proliferation on titanium surfaces, which could be attributed to the improved surface properties.
Highlights
Osseointegration [1], which refers to the process through which the mature living bone establishes a direct structural and functional connection with the implant without any intervening soft or fibrous tissue, is an important phenomenon favoring the effective fixation of implants to bone
There was no significant difference between Ar-Glow discharge plasma (GDP) and GDP-fib groups in blood compatibility analysis
GDP treatment combined with fibronectin grafting favored MG-63 cell adhesion, migration, and proliferation on titanium surfaces, which could be attributed to the improved surface properties
Summary
Osseointegration [1], which refers to the process through which the mature living bone establishes a direct structural and functional connection with the implant without any intervening soft or fibrous tissue, is an important phenomenon favoring the effective fixation of implants to bone. [6,7] Of the different methods, glow discharge plasma (GDP) procedure is a popular method for realizing surface modification of biomaterials. The XPS results showed that the Ti surface after plasma treatment was leaving chemistry uncharged.[11] Most importantly, this method offers the possibility of forming bio-functional groups and functional proteins on the titanium surfaces [12,13]. Given all these advantages, this method can be considered as an efficient tool for improving the biocompatibility of biomaterials.
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