Abstract
ObjectiveTo present a new and effective method of producing titanium surfaces modified with strontium and to investigate the surface characteristics and in vitro biocompatibility of titanium (Ti) surfaces modified with strontium (Sr) for bone implant applications.Materials and MethodsSr-modified Ti surfaces were produced by sequential treatments with NaOH, strontium acetate, heat and water. The surface characteristics and the concentration of the Sr ions released from the samples were examined. Cell adhesion, morphology and growth were investigated using osteoblasts isolated from the calvaria of neonatal Sprague-Dawley rats. Expression of osteogenesis-related genes and proteins was examined to assess the effect of the Sr-modified Ti surfaces on osteoblasts.ResultsThe modified titanium surface had a mesh structure with significantly greater porosity, and approximately5.37±0.35at.% of Sr was incorporated into the surface. The hydrophilicity was enhanced by the incorporation of Sr ions and water treatment. The average amounts of Sr released from the Sr-modified plates subjected to water treatment were slight higher than the plates without water treatment. Sr promoted cellular adhesion, spreading and growth compared with untreated Ti surfaces. The Sr-modified Ti plates also promoted expression of osteogenesis-related genes,and expression of OPN and COL-І by osteoblasts. Ti plates heat treated at 700°C showed increased bioactivity in comparison with those treated at 600°C. Water treatment upregulated the expression of osteogenesis-related genes.ConclusionsThese results show that Sr-modification of Ti surfaces may improve bioactivity in vitro. Water treatment has enhanced the response of osteoblasts. The Sr-modified Ti heat-treated at 700°C exhibited better bioactivity compared with that heated at 600°C.
Highlights
Titanium and its alloys have been widely used in medical applications such as dental and orthopedic implants because of their excellent mechanical properties, osseointegration properties and superior biocompatibility[1, 2].The success rates of dental implants are highly dependent on the quality of the bone-implant interface as well as factors such as patient age, sex, bone quality and site of implantation
The Strontium-Modified Titanium Surface and Osteoblasts. These results show that Sr-modification of Ti surfaces may improve bioactivity in vitro
Water treatment has enhanced the response of osteoblasts
Summary
Titanium and its alloys have been widely used in medical applications such as dental and orthopedic implants because of their excellent mechanical properties, osseointegration properties and superior biocompatibility[1, 2].The success rates of dental implants are highly dependent on the quality of the bone-implant interface as well as factors such as patient age, sex, bone quality and site of implantation. Many drugs have been administered to increase the success rates of dental implants in osteoporotic bone and to enhance osteogenesis after implantation[9, 10, 12, 13].Strontium ranelate (SR) has been successfully used in osteoporotic patients as an anti-osteoporosis drug[14,15,16,17], and studies show that oral administration of SR can improve implant osseointegration and enhance early implant fixation in osteoporotic animals[18, 19] and normal rats[20]. Strontium(Sr) promotes bone formation, and reduces osteoclast activity and bone resorption[21,22,23,24].Long-term local and targeted delivery of an optimal dose Sr to the implant tissue interface is considered a good approach to increase implant osseointegration and avoid the potential adverse effects associated with oral administration[25, 26]. Sr-incorporated Ti oxide surfaces obtained by hydrothermal methods significantly enhanced osteoblast function [28] and promoted biomechanical fixation of implants in a rabbit model[29]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
