In vitro activity of zinc oxide-eugenol and glass ionomer cements on Candida albicans

Anna Carolina Aguiar Cassanho,Claudio Antonio Talge Carvalho,Antonio Olavo Cardoso Jorge,Cristiane Yumi Koga-Ito,Luciane Dias De Oliveira,Aletéia Massula Fernandes

doi:10.1590/s1806-83242005000200011

Anna Carolina Aguiar Cassanho, Claudio Antonio Talge Carvalho + Show 4 more

Open Access

https://doi.org/10.1590/s1806-83242005000200011

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Abstract

The aim of this study was to evaluate in vitro the antimicrobial activity of glass ionomer (GIC) and zinc oxide-eugenol (ZOE) cements against Candida albicans. Standardized GIC and ZOE specimens were maintained in contact with C. albicans suspension (1 x 10(6) cells/ml) at 37 degrees C for 24 h, 48 h or 7 days. A control group without any testing cement was included. After the incubation period, aliquots of 0.1 ml were plated on Sabouraud's agar, and then the number of colonies was counted. The results were expressed as values of logarithms of colony-forming units per milliliter (log CFU/mL) and were analyzed statistically by Kruskal-Wallis ANOVA. After 48 h of incubation, the ZOE group presented no growth of C. albicans. GIC and control groups presented similar mean values at all tested periods. According to the results obtained, it could be concluded that, under the experimental conditions, ZOE cement was more effective in vitro against C. albicans than GIC.

Highlights

Candida genus yeasts are usually isolated from the human oral cavity[21] and their prevalence among healthy individuals varies from 35-38% to 40-60%3,12
After 48 h of incubation, the zinc oxide-eugenol (ZOE) group presented no growth of C. albicans
Counts of Candida albicans after 24 h were lower and statistically different (p = 0.000) in the ZOE group in relation to Glass ionomer cement (GIC) and control groups

Summary

Introduction

Candida genus yeasts are usually isolated from the human oral cavity[21] and their prevalence among healthy individuals varies from 35-38% to 40-60%3,12. C. albicans may be isolated from the mouth of 5-7% of newborns few hours after birth, and from 14.2% of them one week later[17]. C. albicans is the most prevalent species in the mouth (60 to 70% of the isolates), followed by C. tropicalis and C. glabrata[21]. Candida spp. may cause oral and systemic pathologies[6]. The transformation from the saprophytic to the parasitic form is related to microbial, environmental and individual factors[18].

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