Abstract
The growing demand for safe foods without preservatives has fostered research to investigate the effects of natural compounds on microorganisms. Accordingly, an in vitro study was carried out to determine physico-chemical characterization and investigate the antimicrobial effects of Brazil nut oil on toxigenic strains of Aspergillus. The antifungal activity testing was carried out isolating A. parasiticus by transferring the strains to the center of the plates containing treatments with different concentrations of Brazil nut oil. The plates were incubated for 8 days at 28 °C with subsequent extraction of toxins. The aflatoxins AFB1 AFB2, AFG1, and AFG2 were determined by thin-layer chromatography. The values found for acidity, refractive index, and density ranged from 0.3512 to 0.7092, 1.4670 to 1.470, and 9.2 × 102 to 16.7 × 102, respectively. The results obtained showed that the effect of Brazil nut oil on the growth diameter of the fungal colony was time and concentration dependent. After 8 days of incubation, all treatments showed better fungal growth inhibition compared with that of the control. Total inhibition of aflatoxin production by the strain (LOQ of the method: 2 g/kg) was also observed in the medium containing the oil, while in the control treatment, AFB1, AFB2, and AFG1 were produced by the inoculated strain A. parasiticus.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
