In Vicia faba leaves Photoinhibition from Ozone Fumigation in Light Precedes a Decrease in Quantum Yield of Functional PSII Centres

Abstract

Summary Treatment with a single pulse of O3 (150 ppb, 4 h) on Vicia faba (cv. Gigante d’Ingegnoli) plants induced a decrease in photosynthetic rate at light saturation level but only slight changes in stomatal conductance and transpiration rate. The increase in intercellular CO2 concentration implied that the predominant factor responsible for the reduction in light-saturated CO2 uptake was a decrease in the carboxylation efficiency. A slight change in the photochemical efficiency of PS2 (Fv/Fm) that decreased in treated plants more than did the quantum yield of functional centres (ΔF/F′m) lasted hours after treatment termination; photosynthetic efficiency decrease occurred after an increase in F0. Thermal dependence of fluorescence yield of open PS2 reaction centres was modified by O3. This eventually led to photosensitizing these leaves to photoinhibition but quantum yield of functional centres was again less sensitive to this combination. Increase in electrolyte leakage kinetics in distilled water indicated that, in the stressed plants, damage at the membrane level was occurring and this was confirmed also by the thiobarbituric reactive substance increase in ozonated leaves, taken as indicators of membrane lipid peroxidation.