In-situ synthesis of melanin in tumor with engineered probiotics for hyperbaric oxygen-synergized photothermal immunotherapy

Abstract

Anaerobic bacteria and facultative anaerobe are usually used as delivery carriers of antitumor drugs because of their tumor hypoxia targeting and antitumor immune activation abilities, but suffer from dose-dependent systemic side effects. Here, engineered probiotics Nissle 1917 (EcN-T) were constructed for in-situ synthesis of melanin in tumor region for enhanced photothermal therapy (PTT) combining with immunotherapy. By means of genetic engineering, EcN-T that could highly express tyrosinase under near-infrared (NIR) light irradiation were constructed. Due to their intrinsic tumor hypoxia targeting, EcN-T could accumulate and rapidly proliferate in tumor region after intravenous injection. Tyrosinase expression for the following melanin biosynthesis within tumor could be initiated upon NIR exposure. Hyperbaric oxygen treatment facilitated the oxidation of tyrosine to promote the generation of melanin in situ, giving robust photothermal performance to induce immunogenic tumor cell death. Assited by immunostimulatory responses of EcN, maturation of dendritic cells and priming of cytotoxic T cells were largely promoted in tumor tissue, resulting in significant inhibition of tumor growth and recurrence. This bacteria-mediated combined therapeutic strategy showed high efficacy and good safety.