In situ solvent removal-based Eudragit®L/dimethyl sulfoxide forming gel for periodontal pocket drug delivery

Abstract

Periodontitis is a chronic inflammatorydisease of gum resulting from Porphyromonas gingivalis infectious at periodontal pockets. In situ forming gel (ISG) effectively delivers the antimicrobial agents to this targeted site. This study was to develop the dimethyl sulfoxide (DMSO) removal-based ISGs of Eudragit®L and their physicochemical properties were evaluated including the viscosity, rheological behavior, in vitro matrix formation, in vitro doxycycline hyclate (DH) release and antimicrobial activities. The ISGs showed the Newtonian flow which an increase in Eudragit®L amount enhanced a viscosity. They transformed into an opaque gel after exposure a simulated crevicular fluid and modulated the DH release longer than 3 days. The percentage of drug release from DMSO removal-based ISG containing 10, 15, 20 and 25% w/w of Eudragit®L was 95.9, 90.5, 79.8 and 70.0, respectively. Thus, DH release depended on the amount of Eudragit®L loading and corresponded with the polymeric matrix formation. The high Eudragit®L loading formulation retarded notably on the solvent removal owing to a thicker gel barrier. The DH-loaded in situ solvent removal-based Eudragit®L/ DMSO forming gels presented the efficient antimicrobial activities against Staphylococcus aureus, Escherichia coli, Candida albicans and Porphyromonas gingivalis. Therefore, Eudragit®L/DMSO ISG exhibited as a potential periodontal pocket drug delivery system based on solvent removal mechanism.