Abstract
In situ product recovery (ISPR), in the form of an extractive fermentation process, can increase productivity and product titers in the sustainable production of platform chemicals. To establish a guideline for the development of industrially relevant production processes for such bio-based compounds, a wide screening was performed, mapping the potential of an extensive range of solvents and solvent mixtures. Besides solvent biocompatibility with Saccharomyces cerevisiae, distribution coefficients of three organic acids (protocatechuic acid, adipic acid and para-aminobenzoic acid) and four fragrance compounds (2-phenylethanol, geraniol, trans-cinnamaldehyde and β-ionone) were determined. While for highly hydrophobic fragrance compounds, multiple pure solvents were identified that were able to extract more than 98%, reactive extraction mixtures were proven effective for more challenging compounds including organic acids and hydrophilic alcohols. For example, a reactive mixture consisting of 12.5% of the extractant CYTOP 503 in canola oil was found to be biocompatible and showed superior extraction efficiency for the challenging compounds as compared to any biocompatible single solvent. This mapping of biocompatible solvents and solvent mixtures for the extraction of various classes of industrial platform chemicals can be a tremendous step forward in the development of extractive fermentations.
Highlights
The current pressure to shift towards a more sustainable bioeconomy has led to a search in both research and industry for efficient production strategies for bio-based ‘drop-in’ or novel compounds
Given the importance of pH when assessing the extractability of organic acids such as Protocatechuic acid (PCA), adipic acid (AA)
PABA, the pH was adjusted to a value of 4.25 (±0.05) for each of the spiked media
Summary
The current pressure to shift towards a more sustainable bioeconomy has led to a search in both research and industry for efficient production strategies for bio-based ‘drop-in’ or novel compounds. To this end, fermentative production of industrial platform chemicals from second-generation biomass or waste streams could present a principal solution with substantial CO2 abatement potential and a significantly reduced carbon footprint. Efficient platform yeast strains have recently been developed aiming at maximal stress resistance and carbon utilization with second-generation feedstocks [2,3]. Further specific genetic engineering of these platform strains aims to deliver industrial superbugs capable of producing bio-based platform chemicals with great industrial relevance [4]
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