In Situ Precision Cell Electrospinning as An Efficient Stem Cell Delivery Approach for Cutaneous Wound Healing.

Abstract

Mesenchymal stem cell (MSC) therapies have been brought forward as a promising treatment modality for cutaneous wound healing. However, current approaches for stem cell delivery have many drawbacks, such as lack of targetability and cell loss, leading to poor efficacy of stem cell therapy. To overcome these problems, in the present study, wedeveloped an in situ cell electrospinning system as an attractive approach for stem cell delivery. MSCs have a high cell viability over 90% even with a high applied voltage of 15kV post-cell electrospinning process. In addition, cell electrospinning does not show any negative effect on surface marker expression and differentiation capacity of MSCs. In vivo studies demonstrated that in situ cell electrospinning treatment could promote cutaneous wound healing through direct deposition of bioactive fish gelatin fibers and MSCs onto wound sites, leading to a synergic therapeutic effect. Ourapproach enhanced extracellular matrix remodeling by increasing collagen deposition, promoted angiogenesis by increasing the expression of VEGF and forming small blood vessels, and dramatically reduced the expression of IL-6 during the wound healing. The use of in situ cell electrospinning system potentially provides a rapid, no touch, personalized treatment for cutaneous wound healing. This article is protected by copyright. All rights reserved.