In situ measurement of respiratory metabolism and nitrogen fluxes at the interface of oyster beds

Abstract

Nitrogen and oxygen fluxes were measured in situ during monthly dark enclosure experiments on oyster beds. The incubations were performed on undisturbed sediment with its endofauna, on 10 oysters Crassostrea gigas isolated from the substratum, and on 10 oysters associated with the sediment, to assess the impact of oyster farming on the environment. Ammonia was released by the sediment following a seasonal pattern, s ammonia excretion increased in spring (3.21 pm01 g-' h-') and autumn (2.5 to 6.7 pm01 g-' h-'), compared to low winter values (0.28 pm01 g-' h-'), in accordance with changes in temperature and oxygen consumption (0.14 and 1.3 mg O2 g-' h-' in winter and summer respectively). Some nitrate production was observed, suggesting occasional nitrification. Primary amine exchanges were rather erratic, whereas urea seems to play an important role seasonally as a nitrogenous end-product, in relation to a lower condition index at the end of the winter A sirnp!e budge? sf sediment-water exchanges, calculated on a m-' basis (e. g. 2 kg oysters m-'), indicates that over the year the oysters' contribution to the fluxes averages 37 and 40 % for ammonia and urea release respectively, and 26 O/ O for oxygen uptake. However, the actual contribution of oysters to the exchanges at the water-sediment interface rarely fits the expected values from potential estimates. Oxygen consumption, ammonia release and nitrate absorption of the association of oysters with sediment are mainly depressed, except durlng winter and early spring when respiration is stimulated.