Abstract
In bio-catalysis applications, the non-catalytic properties of immobilized matrices have high importance. In this work, we prepared a sodium alginate/polyacrylamide/hydroxyapatite (SA/PAM/HA) hybrid interpenetrating polymer network (HIPN) hydrogel, which we then proved by SEM and FTIR. The HIPN hydrogel exhibited markedly improved mechanical properties. The compression modulus of the HIPN hydrogel (7.16 MPa) was 14 times higher than that of the interpenetrating polymer network (IPN) hydrogel, with the max storage modulus of the HIPN hydrogel being 31,960 Pa. We then employed an extrusion-based bioprinting (3D printing) technique to carry out a one-pot preparation of the immobilized enzymes. Next, we used different types of immobilized glucose oxidase/catalase to synthesize gluconic acid. There was an increase in the catalytic efficiency with the more porous of the immobilized enzymes. Furthermore, the immobilized enzymes exhibited operational stability. Finally, after reuse in 4 batches, a high conversion of 97% was maintained. These results illustrated that the preparation of one-pot immobilized enzymes by 3D bioprinting has great potential for future application.
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