Abstract
In this study, the hemoglobin, egg white, copper phosphate organic–inorganic hybrid nanoflowers (H-E-Cu-NFs), and filter paper-based-egg white-Ca3(PO4)2 HNFs (FP-E-Ca-NFs) were synthesized using a mild biomineralization method. Antibodies (Ab) were labeled on the surface of nanoflowers through highly specific interactions between avidin and biotin. The FP-E-Ca-NFs@Biotin N-hydroxysuccinimide ester–Anti-carcinoembryonic antigen primary antibodies (Biotin-NHS-Ab1)/carcinoembryonic antigen (CEA)/H-E-Cu-NFs@Biotin-NHS-Ab2 immune-biosensing system was constructed by double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) to achieve the visual detection of CEA. Among them, H-E-Cu-NFs have abundant enzyme-like catalytic sites of Fe3+/Cu2+, which can effectively improve the efficiency of the colorimetric reaction. In contrast, E-Ca-NFs have no catalytic sites, and the directional design of catalytic performance realizes the specific identification and signal amplification of CEA detection. The as-developed immune-biosensing system showed a detection range of 0.5 ∼ 100 ng/mL, with the lowest detection limit of 0.5 ng/mL and the CEA recovery between 89.5 % ∼ 108.1 % in human serum samples. Compared to traditional ELISA methods, this immune-biosensing system has the advantages of easy operation, low cost, rapid detection, and on-site detection.
Published Version
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