Abstract
MicroRNAs are highly implicated in an abundance of cellular pathways relevant to human health and disease, including cancer. The critical role miRNAs play in gene regulation and biological function, underscored in their dysregulation in tumor cells and presence as biomarkers in biopsies of cancer patients, warrants further investigation. Current techniques utilized for quantification of miRNAs usually lack adequate sensitivity and quantitative accuracy for rare or hard-to-amplify species. To overcome these pitfalls, we are developing a miRNA single-molecule imaging method for in situ detection by FRET-based probes.
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