Abstract
The phytopathogenic bacteria, Pseudomonas syringae pv. lapsa (P. syringae pv. lapsa) infects the staple food crop wheat. Metalloproteins play important roles in plant-pathogen interactions. Hence, the present work is aimed to predict and analyze the iron (Fe), zinc (Zn), and copper (Cu) binding proteins of P. syringae pv. lapsa which help in its growth, adaptation, survival and pathogenicity. A total of 232 Fe, 307 Zn, and 38 Cu-binding proteins have been identified. The functional annotation, subcellular localization and gene ontology enriched network analysis revealed their role in wide range of biological activities of the phytopathogen. Among the identified metalloproteins, a total of 29 Fe-binding, 31 Zn-binding, and 5 Cu-binding proteins were found to be secreted in nature. These putative secreted metalloproteins may perform diverse cellular and biological functions ranging from transport, response to oxidative stress, proteolysis, antimicrobial resistance, metabolic processes, protein folding and DNA repair. The observations obtained here may provide initial information required to draft new schemes to control microbial infections of staple food crops and will further help in developing sustainable agriculture.
Highlights
Transition metals are essential micronutrients for all living forms principally due to their ability to couple with proteins to form metalloproteins (Bowman et al, 2016)
The proteome scale investigation of P. syringae pv. lapsa for identification of Fe, Zn, and Cu-binding proteins was carried out using the systematic bioinformatics approach (Figure 1)
These shortlisted proteins checked for their subcellular localization which is described in the previous section
Summary
Transition metals are essential micronutrients for all living forms principally due to their ability to couple with proteins to form metalloproteins (Bowman et al, 2016). The interactions between protein and bound prosthetic metal atom in metalloproteins help them to complete the diverse functions of catalysis, regulation and structure stabilization (Andreini et al, 2013; Zhang et al, 2016). The first-row transition metals such as iron (Fe), zinc (Zn), and copper (Cu) are vital for growth and proliferation of most the organisms. Copper is an essential metal micronutrient for structural stabilization of proteins (Kaim and Rall, 1996) and for the catalysis of multiple redox activities of transport, denitrification and oxidative respiration (Cobine et al, 2006; Tavares et al, 2006; Festa and Thiele, 2012). Copper is vital for various metabolic processes related to energy production, melanin biosynthesis and antioxidant defense (Kim et al, 2008)
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