Abstract
BackgroundNucleotide-binding site-leucine-rich repeat (NBS-LRR) resistance genes are the largest class of plant resistance genes which play an important role in the plant defense response. These genes are better conserved than others and function as a recognition-based immune system in plants through their encoded proteins. ResultsHere, we report the effect of Magnaporthe oryzae, the rice blast pathogen inoculation in resistant BR2655 and susceptible HR12 rice cultivars. Transcriptomic profiling was carried out to analyze differential gene expression in these two cultivars. A total of eight NBS-LRR uncharacterized resistance proteins (RP1, RP2, RP3, RP4, RP5, RP6, RP7, and RP8) were selected in these two cultivars for in silico modeling. Modeller 9.22 and SWISS-MODEL servers were used for the homology modeling of eight RPs. ProFunc server was utilized for the prediction of secondary structure and function. The CDvist Web server and Interpro scan server detected the motif and domains in eight RPs. Ramachandran plot of eight RPs confirmed that the modeled structures occupied favorable positions. ConclusionsFrom the present study, computational analysis of these eight RPs may afford insights into their role, function, and valuable resource for studying the intricate details of the plant defense mechanism. Furthermore, the identification of resistance proteins is useful for the development of molecular markers linked to resistance genes.
Highlights
Nucleotide-binding site-leucine-rich repeat (NBS-leucine-rich repeats (LRR)) resistance genes are the largest class of plant resistance genes which play an important role in the plant defense response
Differential gene expression analysis Experiments on disease screening revealed the different stages of resistance between BR2655 and HR12 rice cultivars
BR2655 and HR12 seedlings inoculated with M. oryzae (M036) conidial suspension, showed disease scoring 2, and 8 respectively based on the IRRI SES scale
Summary
Nucleotide-binding site-leucine-rich repeat (NBS-LRR) resistance genes are the largest class of plant resistance genes which play an important role in the plant defense response. These genes are better conserved than others and function as a recognition-based immune system in plants through their encoded proteins. The most important group of genes that have been used by breeders for disease control is the plant resistance (R) genes. Building up a host resistance gene repertoire is of prime concern [2]. Resistance genes (R) are members of a very large multigene family, and these R
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