In Silico Characterization of Resistance Gene Analog, Ds-DbRCaG-05-Rga5p, expressed under Dieback Disease Stress

Abstract

Dalbergia sissoo commonly known as Shisham, is an important timber-producing tree however dieback disease poses considerable challenges to its existence. In this study, the in silico characterization of RGA, Ds-DbRCaG-05-Rga5p, expressed under the dieback challenge was demonstrated. Homology modeling predicted that the identified RGA displayed a similarity with Guanine-N (7)-methyltransferase RID2, an 18S ribosomal RNA protein that plays a role in defense mechanisms in plants. The computational analyses predicted the presence of structural motifs including protein kinase 3, N-glycosylation site, and phosphorylation site that are attributed to proteins containing disease resistance genes. The ligand docking analysis predicted the presence of lysine and arginine residues in active binding sites that suggested its potential as an antimicrobial peptide. Hence, in silico characterization has demonstrated its role in conferring resistance against pathogens.