In Silico Analysis of L-Asparaginase Exposure on Acute Lymphoblastic Leukemia Cell Line RS 4: 11

Abstract

Background The antineoplastic action of Asparaginase (ASNase) was connected to the inability of certain cancer cells to synthesise appropriate level of asparagine (asn). Due to the high affinity of clinically applicable ASNase for asn, asn is rapidly decreased in serum and extracellular fluids following intravenous or intramuscular injection. Tumor cells dependent on external Asn supply and unable of mitigating asn deficiency on their own may starve to death if exogenous asn depletion persists. Methods The current study focused on identifying Differentially Expressed Genes (DEGs) in Acute Lymphoblastic Leukemia (ALL) cell line RS 4;11. A complex biomolecular network for the DEGs was built and various tools were used to compute network parameters and identify drug targets. Results The biomolecular network comprised 109 DEGs. NOTCH1 and PIK3CA may be hub genes and require further research. Conclusion DEGs found could be employed as therapeutic targets for ALL tests or as leads for novel ALL treatments. The current study clarifies the L-ASNase’s exposure on sensitive ALL cell line; resistant cell lines may be investigated further in the future to obtain a greater understanding of ALL.