Abstract
Here, we describe the procedure of a DNA extraction method from maize starch including the method’s validation by in-house and interlaboratory tests. The amplifiable amount of maize DNA tested by real-time PCR was used as parameter for evaluating our method. The practical (i.e. relative) limit of detection (LOD) was used as key criterion for assessing the suitability of the extraction method with respect to genetically modified organism analysis. In a round-robin test with ten participating laboratories, satisfactory results were achieved with practical LODs in the range of 0.1 % with three native maize starch materials. In-house tests showed that this protocol—with an additional purification step—can also be applied for extracting DNA from chemically or enzymatically modified starch.
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