Improving Rabbit Doe Metabolism and Whole Reproductive Cycle Outcomes via Fatty Acid-Rich Moringa oleifera Leaf Extract Supplementation in Free and Nano-Encapsulated Forms.

Abstract

Simple SummaryUnder intensive rabbit production systems, due to the increased energy requirements of reproductive events, specifically pregnancy and lactation, rabbit does may confront several metabolic disorders as a result of energy imbalance. Moringa oleifera leaf ethanolic extract (ME) is one of the phytogenic extracts that has an impressive range of phytochemicals, specifically fatty acids (FAs). These phytochemicals may be biologically effective to support metabolism and reproductive functions of rabbit does during different reproductive cycle events. However, the high FAs content of ME makes them highly susceptible to lipid oxidation, diminishing their nutritional value and biological effects. In this study, we aimed to test the effects of FAs of ME either in a free-from or in a nano-encapsulated form on metabolism, immunity, milk production, milk composition, and reproductive performance of rabbit does during different physiological status (premating, mating, pregnancy, and lactation). The results showed that ME improved health, metabolism, immune functions, milk production and composition, and reproductive performance of rabbit does. These effects remained obvious even when a lower dose of ME was used in a nano-encapsulated form.The effects of free and nano-encapsulated ME supplementations on the metabolism, immunity, milk production and composition, and reproductive performance of rabbit does during premating, mating, pregnancy, and lactation were investigated. Multiparous rabbit does (n = 26 per group) received 50 mg of free ME (FME) daily, 25 mg of nano-encapsulated ME (HNME), or 10 mg of nano-encapsulated ME (LNME) per kilogram of body weight or were not supplemented (C) during a whole reproductive cycle. The ME contained 30 fatty acids with 54.27% total unsaturated fatty acids (USFAs). The fatty acid encapsulation efficiency of alginate nanoparticles was 70.46%. Compared with the C group, rabbits in all ME treatments had significantly increased body weight, feed intake, and glucose concentration and significantly decreased non-esterified free fatty acids and β-hydroxybutyrate concentrations. Rabbits supplemented with ME also had significantly increased white blood cell counts, phagocytic activity, lysozyme activity, and immunoglobulin G and decreased interleukin-1β concentrations. Moreover, ME supplementation significantly increased the concentrations of colostrum immunoglobulins, milk yield and energy content, and milk USFAs (omega-3 and 6). Rabbit does in the ME treatments had significantly higher conception and parturition rates and better litter characteristics than the C rabbit does. These results demonstrate the positive role of ME fatty acids on the health status and productive and reproductive performance of rabbit does at different physiological stages. Compared with the FME treatment, these parameters were further improved in rabbits that received nano-encapsulated ME at lower doses, illustrating how nano-encapsulation technology improves the bioavailability of ME.