Improving catalytic hydrolysis reaction efficiency of sol–gel-encapsulated Candida rugosa lipase with magnetic β-cyclodextrin nanoparticles

Abstract

A silica-based β-cyclodextrin was immobilized on magnetic nanoparticles to obtain a macrocyclic compound with magnetic property. Then, the β-cyclodextrin-grafted magnetic nanoparticles were encapsulated with Candida rugosa lipase in sol–gel matrices using alkoxysilane precursors. The catalytic activity of the encapsulated lipases was evaluated with model reactions, i.e., the hydrolysis of p-nitro-phenylpalmitate (p-NPP) and the enantioselective hydrolysis of rasemic Naproxen methyl ester that was studied in an aqueous buffer solution/isooctane reaction system. The results indicate that the cyclodextrin-based, encapsulated lipase particularly exhibited high conversion and enantioselectivity behavior compared to the sol–gel free lipase. It was also observed that excellent enantioselectivity (E=399) was obtained for the encapsulated lipase with magnetic β-cyclodextrin that has an ee value of S-Naproxen acid of about 98%.