Abstract
Bacopa monnieri (L.) Wettst. or Brahmi has been used in Ayurvedic medicine and currently use as an alternative medicinal herb for several therapeutic healing. Therefore, in vitro mass propagation of B. monnieri for high quality plant production using temporary immersion system (TIS) was necessarily examined. Explants of B. monnieri as regenerated shoots from leaf segments were used to determine the effects of culture system, immersion time and inoculum explant density on growth and bacoside production of B. monnieri. Statistically significant differences in growth and biomass accumulation were observed in the TIS compared with continuous immersion and semi-solid systems. Production of bacoside A, bacopaside II, bacopasaponin C, and total bacoside in B. monnieri obtained from microcontainer cultivation was significantly higher than TIS, whereas total yield of bacosides obtained from different culture systems showed no significant differences. Three immersions per day for 10 min duration promoted growth and biomass accumulation of B. monnieri, while cultivation with 20 explants per container offered optimal inoculum density for large-scale production. Various concentrations and yields of bacoside were obtained from different immersion interval conditions. Reducing immersion duration improved bacoside production. Five explants per container provided the highest bacoside content but yield per container was the lowest.
Published Version
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