Improving arsenic and cadmium contaminated paddy soil health and rice quality with plant-animal-based modified biochar: A mechanistic study

Abstract

Arsenic and cadmium (As/Cd) are toxic elements which negatively impact the health and productivity of rice plants by subjecting them to stress. To address these issues, we developed three biochar amendment materials derived from plant-based corn-stover with iron-magnesium modification (FM@CB), char sourced from animal-based eggshells with iron-magnesium modification (FM@EB), and a blend (1:1, w/w) of both (FM@CEB). Batch adsorption experiment revealed that FM@CB exhibited a Langmuir sorption capacity of 77 mg g−1 for As(III) and 107 mg g−1 for Cd(II). In contrast, FM@EB showed values of 63 mg g−1 for As(III) and 115 mg g−1 for Cd(II), both observed in mixed systems. The exothermic dual adsorption mechanisms could include ion exchange, cation-anion bridging, complexation with surface functional groups, and precipitation processes. Pot trials were conducted, and the findings unveiled a substantial enhancement in rice grain yield with a 2% application of FM@CB, FM@EB, and FM@CEB. Specifically, there was a notable increase of 21.9%, 34.9%, and 37.6%, respectively. Simultaneously, the application led to a reduction in grain As levels by 34.4%, 53.2%, and 42.6%, and grain Cd levels by 48.7%, 71.2%, and 59.9%, respectively, in comparison to the no-amendment control condition. Our study observed a transformation in the soil's microbial community, as beneficial bacterial genera such as Anaeromyxobacter, Citrifermentans, and Desulfovibrio emerged to assist in the detoxification of these metal contaminants. Concurrently, the application of these materials led to a reduction in harmful bacterial genera like Pseudomonas, Flavobacterium, and Massilia. In addition, these amendments decreased the bioavailable As/Cd fractions in the soil and promoted the root-iron-plaque, which effectively sequesters these contaminants, rendering rice quality improvement. Ultimately, the use of these amendments, particularly FM@CEB, holds the promise of protecting rice from harmful metal(loid)s contaminants, increasing yields, and revitalizing the soil. The presence of these unique bacterial genera, especially those capable of detoxifying As/Cd, plays a crucial role in ensuring a healthier and more secure environment for all living organisms.