Improvements in microphotometry by digital signal processing

Abstract

From noise measurements with a microscope photometer it is deduced that the square roots of the measured intensities are linear with respect to photon noise. It is demonstrated by using a cytological specimen with a large dynamic range of intensities, that an IIR filter uniformly smoothes the square roots of the intensities, whereas the effect of that filter on the measured intensities is larger in dark parts of the image than in bright areas.